Izhak Michaelevski, Ph.D.
Building 6, R.6.1.3.b
In 2016, I joined Ariel University as a senior lecturer and principal investigator. My research interest is investigation of molecular and functional mechanisms of memory formation and its impairments by aging related cognitive disorders, as well as understanding fundamental aspects of information processing and storing by the neural system. This is continuation of my research agenda started in 2011 as an independent investigator in Tel Aviv University. During the period of my research activity in Tel Aviv University, my research group made numerous significant discoveries: we identified functional protein expression networks, responsible for memory acquisition and consolidation in hippocampus, as well as for depressive-like behavior; moreover, we found a fundamental mechanism of early phase LTP expression via non-canonical AKT pathway and started decoding of the role of Abl kinase in amyloid beta 42 effect on spontaneous presynaptic release; we also showed correlation between Glur1 overexpression in hippocampus and altered long-term potentiation in depression mice model. In addition, my team designed and implemented an innovative method of in vivo electrophysiological recording from freely moving animal that is one of the most successful and highly appreciate system among the neuroscientists.
My previous projects, before becoming an independent investigator, were not directly related to my current research interests. As in case of my M.Sc and Ph.D. training I investigated biophysical and molecular properties of ion channels, whereas during my post-doctoral research I took advantages of the most advance high-throughput methodology (e.g. mass-spectrometry) in combination with in silico and conventional biochemistry and imaging to elucidate factors impacting peripheral nerve injury related axonal retrograde transport, which "informs" the cell body about the lesion. As a research associate I conducted mastered and conducted very interesting research in structural mass-spectrometry. All these different fields of research opened up new horizons enabling to think out of the box, link advantages of various scientific approaches and study biological phenomena in systemic manner and to be always open minded and easily accepting new ideas and approaches.
The research programs of my lab are very challenging since it endeavors to investigate the Holy Grail of the contemporary neuroscience. This daring attempt entails to employ divergent research methodologies, in many of which I have acquired experience. However since study is never ending for scientists, I consider that strong core of the team and collaboration is the best way to succeed. Strive for taking advantage of multidisciplinary approach strongly affected my choice for my group members. I am lucky that I assembled the core of the group with excellent cooperation with two senior scientists to solve conceptual and technical-methodological challenges. This core creates an excellent conditions for graduate and post-graduate students to learn, acquire experience and conduct research. I encourage cooperation in my lab. Excellence and skills are entry pass to the lab but ability to cooperate is a ticket to stay with us.
In January 2018, I was appointed as a chairman of the Integrative Brain Science Center - Ariel (IBSCA). I consider that advancement of Neuroscience research in Ariel University as my essential goal. My mission as the chairman of IBSCA to enhance existing connection between the neuroscientists in the University, encourage of initiation of new conjoint research project, to establish new collaborative network in the university and between the scientistic of Ariel University and other universitis and research institution in Israel and around the globe. Particular stess will be applied to interdisciplinary collaborations, to link between fundamental research to the applicative aspect of neuroscience, including interaction with medical branches and industry. The center will endeavor to bring the neuroscience research in Ariel University to the highest level as conducted in the top-notch universities. Beyond research projects, preparing and training of the future neuroscientists will executed by implementing of new educational advanced neuroscience study program. IBSCA will turn to the center of knowledge and experience sharing and dissemination, annual international conferences, weekly seminars of invited outstanding scientists will further broaden horisons of the students, post-doctoral fellows and investigators in the university. The center will also serve as a source of knowledge dissemination to the general population and conduct multiple social activities . It is very exciting time to be in the frontiers of neuroscience in Ariel University these times.
KNOWLEDGE BEYOND MY RESEARCH:
Sometimes it is very difficult to decide what to study since there are so much interesting things around us. Consequently, if we cannot learn everything fundamentally, we can for a personal interest to get deeper into different fields of science. So my interests involve:
Theoretical physics, namely, theory of superstrings, M-theory, theory of quantum gravitation and so on...
Computers: principles of operating systems functioning; network security
Geology and tectonics
History of the ancient world and medieval times
And some other small things...........
Neither scientist can be good enough if he works all the time. Permanent load is supposed to make us less creative and more technical, devoid ideas with limited horizon of understanding of things. Leisure is supposed to restart our biological computer, throw out garbage of unsuccessful ideas, replenish our mind and refresh our ability to work fruitfully further. If you do not think so, you lack imagination of scientist.
Hence, I have a lot of interests beyond the knowledge:
Hiking, rappelling, rafting, diving, martial arts, traveling, music, photography and an additional long list not to be shown....
Introduction to Molecular Neurobiology (Syllabus )
Introduction to Physiology, section B (Syllabus -LINK)
Molecular Mechanisms of Psychiatric Disorders (with Prof. Pinhasov, (Syllabus -LINK))
Signal Transduction (Syllabus -LINK)
Biochemistry – Introduction to Metabolism (Syllabus -LINK)
Advanced Laboratory Methods (Syllabus -LINK)
Neuroscience for Neuroscientists (Syllabus)
Cited by 865 (for updated info: Google Scholar)
h-index = 15
1) Fili O*,Michaelevski I*,Bledi Y, Chikvashvili D, Singer-Lahat D, Boshwitz H, Linial M, Lotan I. Direct interaction of a brain voltage-gated K+ channel with syntaxin 1A: functional impact on channel gating. J Neurosci 2001 Mar15; 21(6):1964-74.
2) Michaelevski I, Chikvashvili D,Tsuk S, Fili O,Lohse MJ, Singer-LahatD, Lotan I. Modulation of a brain voltage-gated K channel by syntaxin1A requires the physical interaction of Gbeta/gamma with the channel. J BiolChem 2002 Sep20; 277(38):34909- 17.
3) Michaelevski I,Chikvashvili D,Tsuk S, Singer-Lahat D, Kang Y, Linial M, Gaisano HY,Fili O, Lotan I. Direct interaction of target SNAREs with the Kv2.1 channel. Modal regulation of channel activation and inactivation gating. J Biol Chem. 2003 Sep 5; 278(36):34320-30.
4) Tsuk S*,Michaelevski I*,Bentley GN, Joho RH, Chikvashvili D, Lotan I. Kv2.1 channel activation and inactivation is influenced by physical interactions of both syntaxin1A and the t- SNARE complex with the C-terminus of the channel. Mol Pharmacol. 2005 Feb;67(2): 480-8.
5) Wolf-Goldberg T*,Michaelevski I*,Sheu L, Gaisano HY, Chikvashvili D, Lotan I. Target soluble N-ethylmaleimidesensitive factor attachment protein receptors(t-SNAREs) differently regulate activation and inactivation gating of Kv2.2 and Kv2.1: Implications on pancreatic islet cell Kv channels. MolPharmacol. 2006 Sep;70(3):818-28.
6) Michaelevski I,Korngreen A, Lotan I. Interaction of syntaxin with a single Kv1.1 channel: a possible mechanism for modulating neuronal excitability. Pflugers Arch.2007 Jun; 454(3):477-94.
7) Perlson E,Michaelevski I, Kowalsman N, Ben-Yaakov K, Shaked M,Seger R, Eisenstein M, Fainzilber M. Vimentin binding to phosphorylated erk sterically hinders enzymatic dephosphorylation of the kinase. J Mol Biol. 2006 Dec15; 364(5):938-44.
8) Lvov A, Chikvashvili D, Michaelevski I, Lotan I. VAMP2 interacts directly with the N Terminus of Kv2.1 to enhance channel inactivation. Pflugers Arch. 2008 Sep; 456(6):1121-36.
9) Tsuk S, Lvov A, Michaelevski I, Chikvashvili D, Lotan I. Formation of the full SNARE complex eliminates interactions of its individual protein components with the Kv2.1 channel. Biochemistry.2008 Aug 12; 47(32):8342-9.
10) Feinshreiber L, Chikvashvili D, Michaelevski I, Lotan I. Syntaxin modulates Kv1.1 through dual action on channel surface expression and conductance. Biochemistry. 2009 May 19; 48(19):4109-14
11) Lvov A, Greitzer D, Chikvashvili D,Tsuk S, Berlin S,Lotan I& and Michaelevski I& Rearrangement in the relative orientation of cytoplasmic domains induced by a membrane anchored protein–mediated Kv channel gating modulation. JBiol Chem 2009, 284(41):28276-91.
12) Rishal I,Michaelevski I, Shinder V, Medzihradszky KF, Burlingame AL, Fainzilber M Axoplasm Isolation from Peripheral Nerve. Developmental Neurobiology, 2010 Feb;70(2):126-33;
13) Michaelevski I&,Medzihradszky KF, Burlingame,AL & Fainzilber M&: Axonal transport proteomics reveals mobilization translation machinery to the lesion site in injured sciatic nerve. Mol.Cell.Proteomics. 2010 May 9 (5):976-87.
14) Michaelevski I, Segal-Ruder Y, Rozenbaum M, Medzihradszky KF, Shalem O, Coppola G, Horn-Saban S, Ben-Yaakov K, Dagan SY, Rishal I, Geshwind DH,Pilpel Y, BurlingameAL, Fainzilber M. Signaling to Transcription Networks in the Neuronal Retrograde Injury response. Science Signaling. 2010 Jun13; 3(130):ra53;
15) Michaelevski I, Eisenstein M, Sharon M: Gas-phase compaction and unfolding of protein structures. AnalyticalChemistry 2010 Nov 15; 82(22): 9484-91.
16) Kirshenbaum N, Michaelevski I, Sharon M. Analyzing Large Protein Complexes by Structural Mass Spectrometry. Journal of Visualized Experiments, 2010; ePublish: http://www.jove.com/index/Details.stp?ID=1954; Review
17) Michaelevski I, Kirshenbaum N, Sharon M. T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis. Journal of Visualized Experiments, 2010; ePublish: http://www.jove.com/index/details.stp?id=1985;Review
18) Michaelevski I, Lotan I. Role of Neuronal Potassium M Channels in Sympathetic Regulation of Cardiac Function. Journal of Physiology, 2011 Jun 1; 589(Pt 11):2659-60. Review
19) Moscovitz O, Tsvetkov P, Hazan N, Michaelevski I, Keisar H, Ben-Nissan G, Shaul Y, Sharon M. A Mutually Inhibitory Feedback Loop between the 20S Proteasome and Its Regulator, NQO1. Molecular Cell 2012 Jul 13; 47(1):76-86.
20) Cho Y, Liberto V D, Carlin D, Abe N, Burlingame A L, Li K H , Guan S, Michaelevski I, and Cavalli V, The SNARE protein syntaxin13 is an mTOR translational target promoting axon regeneration. J Biol Chem. 2014 May 30;289 (22):15820-32.
21) Sheinin A, Lavi A, Michaelevski I&. StimDuino: an Arduino-based electrophysiological stimulus isolator. Journal of Neuroscience methods. 2015 Mar 30; 243:8-17. doi: 10.1016/j.jneumeth.2015.01.016.
22) Gross M, Sheinin A, Nesher E, Tikhonov T, Baranes D, Pinhasov,A, Michaelevski I& Early onset of cognitive impairment is associated with altered synaptic plasticity and enhanced hippocampal GluA1 expression in a mouse model of depression Neurobiology of aging. 2015 May;36(5):1938-52. doi: 10.1016/j.neurobiolaging.2015.02.015.
23) Nesher E, Koman, I, Gross M, Tikhonov T, Bairachnaya M, Gerliz G, Michaelevski I, Yadid G, Pinhasov A. Synapsin IIb as a functional marker of submissive behavior Scientific Reports 2015 May 22; 5:10287. doi: 10.1038/srep10287.
24) Borovok N, Nesher E, Levin Y, Reichenstein M, Pinhasov A, Michaelevski I&, Dynamics of hippocampal protein expression during long-term spatial memory formation. Molecular and cellular proteomics 2016 Feb;15(2):523-41. doi: 10.1074/mcp.M115.051318.
25) Chandran V, Coppola G, Omura T, Nawabi H, Versano R, Costigan M, Blesch A, Michaelevski I, Davis-Turak J, Gao F, Langfelder P, Horvath S, He Z, Fainzilber M, Tuszynski M, Woolf C J, Geschwind D, A Systems-Level Analysis of the Peripheral Nerve Intrinsic Axonal Growth Program Neuron 2016, Mar 2; 89, 1-15.
26) Pen Y, Borovok N, Sheinin A, Reichenstein M, Michaelevski I& The role of membrane anchored AKT in regulation of basic synaptic transmission and early phase LTP expression. Hippocampus 2016: Sep;26(9):1149-67; Apr 12; doi: 10.1002/hipo.22597; PMID: 27068236 (Epub ahead of print)
27) Borovok N, Nesher E, Reichenstein M, Levin Y, Tikhonova T, Pinhasov A and Michaelevski I.& Hippocampal protein expression alteration in depression mice models in naïve vs. stress conditions Molecular and cellular proteomics. Proteomics Clin Appl. 2017 Jul 5. doi: 10.1002/prca.201700089.
28) Ganz J, Cohen-Matsliah SI, Shor E, Sheinin A, Arie I, Michaelevski I, Pitaru S, Offen D and Levenberg S. Implantation of 3-D constructs embedded with oral mucosa-derived cells induces functional recovery in rats with complete spinal cord transection. Frontiers in Neuroscience (Accepted, manuscript ID: 289724).
29) Singer-Lahat D, Barak-Broner N, Sheinin A, Gretzer-Antes D, Michaelevski I and Lotan I The dual function of the polybasic juxtamembrane region of syntaxin 1A in clamping spontaneous release and stimulating Ca2+-triggered neurotransmitter release in neuroendocrine cells. J Neuroscience 2017 (Accepted, manuscript ID: JN-RM-1541-17R1)
30) Yanovich C, Kirby M, Michaelevski I, Yadid G, Pinhasov A Critical role of personality in stress-induced drug dependency. Scientific Reports 2018 (Accepted SREP-17-42744A)
*- equal contributor
&- corresponding author
On Revision Manuscripts: (total: 3)
31) Reichenstein M, Sheinin A, Borovok N, Michaelevski I&. Abl Kinase mediates spontaneous synaptic activity depression induced by Amyloid Beta 1-42 peptides (Pending on revision, Neurobiology of Aging)
32) Panayotis N, Dagan S, Sheinin A, Rother F, Hartmann E, Bader M, Michaelevski I, Fainzilber M#. Deciphering Importin-a Roles in Mammalian Central Neurons. Neuron (pending on revision)
Articles in the final stage of preparation*: (currently: 9)
33) Reichenstein M, Nesher E, Borovok N, Levin Y, Tikhonova T, Pinhasov A, Michaelevski I& Protein expression profiles changes in striatum during acquisition of the reference spatial memory (in preparation).
34) Nesher E, Borovok N, Levin Y, Tikhonova T, Pinhasov A, Michaelevski I& Striatal protein expression alteration in depression mice models in naïve vs. stress conditions (in preparation).
35) Reichenstein M, Sheinin A, Borovok N, Michaelevski I& Abl2 kinase effect on post-synaptic neuronal activity is mediated by regulation of AMPA receptor current. (in preparation).
36) Bairachnaya M, Sheinin A, Pinhasov A, Michaelevski I& Submissiveness is associated with altered brain electrical activity on EEG. (In preparation)
37) Reichenstein M, Sheinin A, Borovok N, Michaelevski I& Abl kinase regulates reference spatial memory and late phase synaptic plasticity in hippocampus. (in preparation)
38) Michaelevski M Non-deterministic quantum-like behavior of neural system: neurocode vs neural energetic states. (In preparation)
39) Bairachnaya M, Michaelevski I, Pinhasov A Link between stress sensitivity and aging (In preparation)
40) Bairachanaya M, Reichenstein M, Gitler D, Michaelevski I., Pinhasov A Role of synapsin2b expression in stress-sensitivity related cognitive alteration (In preparation).
41) Brider T, Reichenstein M, Michaelevski I&. Narrow range of terahertz radiation band selectively reduces survival of cancer cell lines in vitro.
*-titles of manuscripts in preparation may be subject of modification prior submission/publication
#-list of the authors is not final
Book Chapters (total: 2)
Michaelevski I, and Fainzilber M. 2007: Proteomic approaches to axon injury–Post- genomic approaches to a posttranscriptional process. IN: Neural Degeneration and Repair–Gene Expression Profiling, Proteomics, Glycomics and Systems Biology. Editor Werner-Mueller H. 2008 Wiley-VCH Verlag GmbH &Co. KGaA
Pinhasov A, Michaelevski I, Koman I, Nesher E PACAP and Depression. IN: Pituitary Adenylate Cyclase Activating Polypeptide – PACAP. Editor Dora Reglodi and Andrea Tamas. In Press.