ABOUT ME

I joined the laboratory of Dr. Michaelevski in May 2012. Currently, I am focused on investigating the effects of molecular interventions with Abl kinase activity on synaptic transmission in cultured hippocampal neurons employing the patch clamp technique, as well as various molecular biology methods.
Outside the laboratory my other great passion is the piano. I began my classical training at a very young age, and never stopped playing since.

 

MY PROJECT

Role of ABL tyrosine kinase in establishing of late phase LTP
Abelson (Abl) family non-receptor tyrosine kinases have multiple regulatory roles in the developing and mature CNS, including coordination of cytoskeleton remodeling, clustering of post-synaptic density proteins and modulation of NMDA receptor activity.
My project aim is to investigate the possible role of Abl kinases in long-term synaptic plasticity and memory formation, employing electrophysiological, biochemical and behavioral approaches.

 

PUBLICATIONS

• Reichenstein M., Rehavi M., Pinhasov A. Involvement of Pituitary Adenylate Cycalse Activating Polypeptide (PACAP) and its Receptors in the Mechanism of Antidepressant Action J Mol Neurosci 2008;36(1-3):330-8

• Borovok N, Nesher E, Levin Y, Reichenstein M,  Pinhasov A, Michaelevski I&, Time dependent protein expression alteration occurring in hippocampus during in formation of long-term spatial memory.  Molecular and cellular proteomics http://www.ncbi.nlm.nih.gov/pubmed/26598641; pii: mcp.M115.051318

• Pen Y, Borovok N, Sheinin A, Reichenstein M, Michaelevski I The role of membrane anchored AKT in regulation of basic synaptic transmission and early phase LTP expression.  Hippocampus 2016; Sep;26(9):1149-67.; doi: 10.1002/hipo.22597; PMID: 27068236

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• Reichenstein M, Sheinin A, Borovok N, Michaelevski I&.  Abl Kinase mediates spontaneous synaptic activity depression induced by Amyloid Beta 1-42 peptides (Neurobiology of Aging, pending on revision).

• Borovok N, Nesher E, Reichenstein M, Levin Y, Tikhonova T, Pinhasov A and Michaelevski I.& Hippocampal protein expression alteration in depression mice models in naïve vs. stress conditions Molecular and cellular proteomics (Pending on revision, MCP).

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CURRICULIM VITAE

A. EDUCATION

2012 -          Ph.D. student at the Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences under the supervision of Dr. Izhak Michaelevski. Research project theme : Characterization of the role of Ableson kinase in long-term synaptic plasticity and memory formation

2005 - 2008 M.Sc. in Medical Sciences, Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Thesis theme: "Involvement of Pituitary Adenylate Cycalse Activating Polypeptide (PACAP) and its Receptors in the Mechanism of Antidepressant Action " Supervisors: Prof. Moshe Rehavi and Dr. Albert Pinhasov

2001 - 2004 B.Sc. in Life Sciences, Faculty of Life Science, Bar-Ilan University, Israel. Research project under the supervision of Prof. Y. Nitzan and Dr.Z Leizerovitch, Clinical Microbiology, Assaf Harofe Medical Center, Zerifin, Israel 

1996 -          High school Graduate, specialization in biology and chemistry, Aharon Katzir High school, Rehovot, Israel

 

B. FORMER OCCUPATION

2009 - 2012  Laboratory technician at the department of Molecular Genetics at Maccabi Healthcare Services – Megalab, Rehovot

 

C. LABORATORY EXPERIENCE

• Establishment of rat primary cortical and hippocampal neuronal cell cultures

• Dissection techniques for retrieval of hippocampal and cortical tissues from rodent brains

• Cell-line culturing (CHO, PC-12, NB-1)

• Highly experienced in various molecular biology methods:

• DNA, RNA and protein extraction (cell cultures/tissues)

• PCR, RT-PCR

• Real-time PCR (SYBR, TaqMan)

• Western blotting and immunohistochemistry

• DNA sequencing and fragment analysis by Capillary Electrophoresis

 

D. ADDITIONAL QUALIFICATIONS

07.2010 – Certificate of Recognition as an academic medical lab technician issued by the Ministry of Health, Israel

2006-2007 - Teaching assistant at biochemistry and microbiology laboratory courses; Department of Molecular Biology, Ariel University Center of Samaria, Israel. Computers: Microsoft Office, Endnote8, GraphPad Prism 5.0 (Statistical analysis and graphic presentation)

languages: fluent at English, Hebrew and Russian

1996 – 1998 – Full term military service